Rat SPA(Surfactant Associated Protein A) ELISA Kit

SKU: ELK11084

Size:96T

Reactivity:Rat

Alternative Names:PRL; SFTPA; PSAP; PSPA; SFTP; SFTPA1; COLEC4; PSP-A; SFTP1; SFTPA1B; SP-A1; Collectin-4; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein

Assay Type:Sandwich

Sensitivity:0.17 ng/mL

Standard:50 ng/mL

Detection range:0.78-50 ng/mL

Sample type:serum, plasma, tissue homogenates, lung lavage fluid and other biological fluids

Assay length:3.5h

Research Area:Infection immunity;Pulmonology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SPA in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P08427