Rat SDC1(Syndecan 1) ELISA Kit

SKU: ELK5830

Size:96T

Reactivity:Rat

Alternative Names:CD138; SDC; SYND1; Syndecan Proteoglycan 1

Assay Type:Sandwich

Sensitivity:0.54 ng/mL

Standard:100 ng/mL

Detection range:1.57-100 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Tumor immunity;Infection immunity;Immunodeficiency;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SDC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SDC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SDC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SDC1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P26260