Rat Pro-ANP(Pro Atrial Natriuretic Peptide) Microsample ELISA Kit

SKU: ELK8360MS

Size:96T

Reactivity:Rat

Alternative Names:Pro-ANP

Assay Type:Sandwich

Sensitivity:15.2 pg/mL

Standard:4000 pg/mL

Detection range:62.5-4000 pg/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Endocrinology;Cardiovascular biology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Pro-ANP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P01161