Rat MxA(Myxovirus resistant A) ELISA Kit

SKU: ELK11791

Size:96T

Reactivity:Rat

Alternative Names:MxA

Assay Type:Sandwich

Sensitivity:0.192 ng/mL

Standard:100 ng/mL

Detection range:1.57-100 ng/mL

Sample type:serum, plasma

Assay length:3.5h

Research Area:Immunology & Inflammation;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MxA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MxA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MxA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MxA in the samples is then determined by comparing the OD of the samples to the standard curve.

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