Rat FAS(Factor Related Apoptosis) ELISA Kit

SKU: ELK10431

Size:96T

Reactivity:Rat

Alternative Names:CD95; ALPS1A; ALPS1-A; APO1; APT1; FAS1; FASTM; TNFRSF6; Fas Receptor; TNF Receptor Superfamily Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6

Assay Type:Sandwich

Sensitivity:0.098 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Assay length:3.5h

Research Area:Apoptosis;Tumor immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FAS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FAS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FAS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FAS in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q63199