Rat Cholesterol side-chain cleavage enzyme, mitochondrial,Cyp11a1 ELISA KIT

SKU: E2618Ra

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). {ECO:0000250|UniProtKB:P05108}.

Research_area:

Applications:ELISA

Target_protein:Cyp11a1

Reactivity:Rat

Assay type:Sandwich

Sensitivity:11.63ng/L

Detection range:25-1600ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cytochrome P450 Side Chain Cleavage Enzyme

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P14137

MASS(Da):60586

GeneID:29680

Synonyms:Cyp11a1 Cyp11a Cyp11a-1

Gene names:Cyp11a1

Standard solution:3200ng/L