Rat C4a(Complement Component 4a) Microsample ELISA Kit

SKU: ELK11116MS

Size:96T

Reactivity:Rat

Alternative Names:C4a anaphylatoxin

Assay Type:Sandwich

Sensitivity:0.05 µg/mL

Standard:10 µg/mL

Detection range:0.16-10 µg/mL

Sample type:serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Immune molecule;Endocrinology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C4a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C4a in the samples is then determined by comparing the OD of the samples to the standard curve.

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