Rat 6ketoPGF1α(6-keto-PGF1 alpha) ELISA Kit

SKU: ELK8363

Size:96T

Reactivity:Rat

Alternative Names:6-keto-PGF1 alpha; 6ketoPGF1α

Assay Type:Competitive Inhibition

Sensitivity:1.4 pg/mL

Standard:500 pg/mL

Detection range:7.8-500 pg/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Neuro science;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 6ketoPGF1α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 6ketoPGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 6ketoPGF1α in the samples is then determined by comparing the OD of the samples to the standard curve.

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