Rat 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial,CYP24A1 ELISA KIT

SKU: E1173Ra

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24-oxidation pathway, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)) (PubMed:2026586, PubMed:16617161, PubMed:10231362). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed:10231362, PubMed:16617161). Hydroxylates at C-24 or C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol (PubMed:25727742). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed:2026586). {ECO:0000269|PubMed:10231362, ECO:0000269|PubMed:16617161, ECO:0000269|PubMed:2026586, ECO:0000269|PubMed:25727742}.

Research_area:

Applications:ELISA

Target_protein:Cyp24a1

Reactivity:Rat

Assay type:Sandwich

Sensitivity:0.049ng/ml

Detection range:0.1-35ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q09128

MASS(Da):59448

GeneID:25279

Synonyms:Cyp24a1 Cyp24

Gene names:Cyp24a1

Standard solution:40ng/ml