RAG2 Antibody, FITC conjugated - Cat. #: CSB-PA345128LC01HU

SKU: CSB-PA345128LC01HU

Size :50ug

Clone Number:

Aliases:RAG 2 antibody; RAG-2 antibody; RAG2 antibody; RAG2_HUMAN antibody; Recombination activating gene 2 antibody; V(D)J recombination activating protein 2 antibody; V(D)J recombination-activating protein 2 antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P55895

Immunogen:Recombinant Human V(D)J recombination-activating protein 2 protein (296-415AA)

Raised in:Rabbit

Species Reactivity:Human

Tested Applications:

Background:Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3\'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5\'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at \'Lys-4\' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity).

Clonality:Polyclonal

Isotype:IgG

Purification Method:>95%, Protein G purified

Conjugate:FITC

Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form:Liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:RAG2

Research Areas:Epigenetics and Nuclear Signaling