Rabbit SREBP1(Sterol regulatory element-binding protein 1) ELISA Kit

SKU: ELK9798

Size:96T

Reactivity:Rabbit

Alternative Names:SREBF1; BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1

Assay Type:Sandwich

Sensitivity:0.059 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Cardiovascular; Epigenetics and Nuclear Signaling; Cancer; Metabolism

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit SREBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit SREBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit SREBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit SREBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q9MYY5