Rabbit sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit

SKU: ELK11213

Size:96T

Reactivity:Rabbit

Alternative Names:MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9

Assay Type:Sandwich

Sensitivity:12.8 ng/mL

Standard:2000 ng/mL

Detection range:31.25-2000 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Immunology；Metabolism；Signal Transduction

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: