Purified Rabbit anti-WNT1 Antibody

SKU: DL91232A

Size 1: 100 ul

Synonyms : INT1; Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Mk, P

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to WNT1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human WNT1. The exact sequence is proprietary.

WB description: Western blot analysis of WNT1 expression in HEK293T (A), SP2/0 (B), H9C2 (C), WI38 (D), NIH3T3 (E) whole cell lysates.

IHC description: Immunohistochemical analysis of WNT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of WNT1 staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 7471

Gene ID (Mouse): 22408

Gene ID(Rat):

SwissProt (Human): P04628

SwissProt (Mouse): P04426

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.