Purified Rabbit anti-UPF1 Antibody

SKU: DL99312A

Size 1: 100 ul

Synonyms : KIAA0221; RENT1; Regulator of nonsense transcripts 1; ATP-dependent helicase RENT1; Nonsense mRNA reducing factor 1; NORF1; Up-frameshift suppressor 1 homolog; hUpf1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IH

Background: Rabbit polyclonal antibody to UPF1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human UPF1. The exact sequence is proprietary.

WB description: Western blot analysis of UPF1 expression in CT26 (A), PC3 (B), MCF7 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of UPF1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)

Gene ID(Human): 5976

Gene ID (Mouse): 19704

Gene ID(Rat):

SwissProt (Human): Q92900

SwissProt (Mouse): Q9EPU0

SwissProt (Rat):

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.