Purified Rabbit anti-TNF alpha Antibody

SKU: DL91174A

Size 1: 100 ul

Synonyms : TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Mk, P

Applications: WB, IHC

Background: Rabbit polyclonal antibody to TNF alpha

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TNF alpha. The exact sequence is proprietary.

WB description: Western blot analysis of TNF alpha expression in HeLa (A), NIH3T3 (B), mouse heart (C), rat brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 7124

Gene ID (Mouse): 21926

Gene ID(Rat): 24835

SwissProt (Human): P01375

SwissProt (Mouse): P06804

SwissProt (Rat): P16599

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.