Purified Rabbit anti-TCP1 alpha Antibody

SKU: DL97639A

Size 1: 100 ul

Synonyms : CCT1; CCTA; T-complex protein 1 subunit alpha; TCP-1-alpha; CCT-alpha

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to TCP1 alpha

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human TCP1 alpha

WB description: Western blot analysis of TCP1 alpha expression in PC3 (A), Hela (B), Jurkat (C) whole cell lysates.

IHC description: Immunohistochemical analysis of TCP1 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of TCP1 alpha staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 6950

Gene ID (Mouse): 21454

Gene ID(Rat): 24818

SwissProt (Human): P17987

SwissProt (Mouse): P11983

SwissProt (Rat): P28480

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.