Purified Rabbit anti-SRP19 Antibody

SKU: DL96329A

Size 1: 100 ul

Synonyms : Signal recognition particle 19 kDa protein; SRP19

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to SRP19

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human SRP19

WB description: Western blot analysis of SRP19 expression in HepG2 (A), MCF7 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of SRP19 staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of SRP19 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/10 - 1/100)

Gene ID(Human): 6728

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P09132

SwissProt (Mouse): Q9D7A6

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.