Purified Rabbit anti-SMAD1 (pS187) Antibody

SKU: DL90704A

Size 1: 100 ul

Synonyms : BSP1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, C, S

Applications: WB, IHC, IP

Background: Rabbit polyclonal antibody to SMAD1 (pS187)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMAD1. The exact sequence is proprietary.

WB description: Western blot analysis of SMAD1 (pS187) expression in HEK293T BMP4-treated (A), HeLa PMA-treated (B), A673 (C), PC12 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of SMAD1 (pS187) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Gene ID(Human): 4086

Gene ID (Mouse): 17125

Gene ID(Rat):

SwissProt (Human): Q15797

SwissProt (Mouse): P70340

SwissProt (Rat): P97588

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.