Purified Rabbit anti-RPS10 Antibody

SKU: DL97075A

Size 1: 100 ul

Synonyms : 40S ribosomal protein S10

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to RPS10

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human RPS10

WB description: Western blot analysis of RPS10 expression in A549 (A), HT29 (B), mouse spleen (C), mouse heart (D) whole cell lysates.

IHC description: Immunohistochemical analysis of RPS10 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of RPS10 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/20 - 1/50), IP (1/20 - 1/50)

Gene ID(Human): 6204

Gene ID (Mouse): 67097

Gene ID(Rat): 100363439; 81773

SwissProt (Human): P46783

SwissProt (Mouse): P63325

SwissProt (Rat): P63326

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.