Purified Rabbit anti-RNF125 Antibody

SKU: DL93205A

Size 1: 100 ul

Synonyms : E3 ubiquitin-protein ligase RNF125; RING finger protein 125; T-cell RING activation protein 1; TRAC-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, Mk

Applications: WB, IHC

Background: Rabbit polyclonal antibody to RNF125

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RNF125. The exact sequence is proprietary.

WB description: Western blot analysis of RNF125 expression in HEK293T (A), THP1 (B), 129Sv (C) whole cell lysates.

IHC description: Immunohistochemical analysis of RNF125 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 54941

Gene ID (Mouse): 67664

Gene ID(Rat):

SwissProt (Human): Q96EQ8

SwissProt (Mouse): Q9D9R0

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.