Purified Rabbit anti-RhoBTB1 Antibody

SKU: DL98863A

Size 1: 100 ul

Synonyms : RHOBTB1; KIAA0740; Rho-related BTB domain-containing protein 1; RHOBTB2; DBC2; KIAA0717; Rho-related BTB domain-containing protein 2; Deleted in breast cancer 2 gene protein; p83

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Mk

Applications: WB, IH, IF/IC

Background: Rabbit polyclonal antibody to RhoBTB1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human RhoBTB1. The exact sequence is proprietary.

WB description: Western blot analysis of RhoBTB1 expression in CT26 (A), C6 (B), HEK293T (C), SGC7901 (D), U87MG (E) whole cell lysates.

IHC description: Immunohistochemical analysis of RhoBTB1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of RhoBTB1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 23221; 9886

Gene ID (Mouse): 69288; 246710

Gene ID(Rat):

SwissProt (Human): O94844; Q9BYZ6

SwissProt (Mouse): Q9DAK3; Q91V93

SwissProt (Rat):

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.