Purified Rabbit anti-PTEN (pS385) Antibody

SKU: DL94742A

Size 1: 100 ul

Synonyms : MMAC1; TEP1; Phosphatidylinositol 345-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, D, Mk, P

Applications: WB, IHC

Background: Rabbit polyclonal antibody to PTEN (pS385)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PTEN. The exact sequence is proprietary.

WB description: Western blot analysis of PTEN (pS385) expression in A549 (A), THP1 (B), mouse spleen (C), Raw264.7 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of PTEN (pS385) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100)

Gene ID(Human): 5728

Gene ID (Mouse): 19211

Gene ID(Rat):

SwissProt (Human): P60484

SwissProt (Mouse): O08586

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.