Purified Rabbit anti-PSMD8 Antibody

SKU: DL96363A

Size 1: 100 ul

Synonyms : 26S proteasome non-ATPase regulatory subunit 8; 26S proteasome regulatory subunit RPN12; 26S proteasome regulatory subunit S14; p31

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to PSMD8

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human PSMD8

WB description: Western blot analysis of PSMD8 expression in Jurkat (A), SW480 (B), mouse testis (C), mouse thymus (D) whole cell lysates.

IHC description: Immunohistochemical analysis of PSMD8 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PSMD8 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 5714

Gene ID (Mouse): 57296

Gene ID(Rat):

SwissProt (Human): P48556

SwissProt (Mouse): Q9CX56

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.