Purified Rabbit anti-PPAN Antibody

SKU: DL98186A

Size 1: 100 ul

Synonyms : BXDC3; SSF1; Suppressor of SWI4 1 homolog; Ssf-1; Brix domain-containing protein 3; Peter Pan homolog

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to PPAN

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human PPAN

WB description: Western blot analysis of PPAN expression in HepG2 (A), mouse heart (B) whole cell lysates.

IHC description: Immunohistochemical analysis of PPAN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PPAN staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 56342

Gene ID (Mouse): 235036

Gene ID(Rat):

SwissProt (Human): Q9NQ55

SwissProt (Mouse): Q91YU8

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.