Purified Rabbit anti-PLC beta 3 Antibody

SKU: DL98809A

Size 1: 100 ul

Synonyms : 1-phosphatidylinositol 45-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IH, IF/IC

Background: Rabbit polyclonal antibody to PLC beta 3

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PLC beta 3. The exact sequence is proprietary.

WB description: Western blot analysis of PLC beta 3 expression in HCT116 (A), Beas2B (B), PMVEC (C), CT26 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of PLC beta 3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PLC beta 3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 5331

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): Q01970

SwissProt (Mouse): P51432

SwissProt (Rat): Q99JE6

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.