Purified Rabbit anti-PKC alpha (pT638) Antibody

SKU: DL93782A

Size 1: 100 ul

Synonyms : PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IHC

Background: Rabbit polyclonal antibody to PKC alpha (pT638)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PKC alpha. The exact sequence is proprietary.

WB description: Western blot analysis of PKC alpha (pT638) expression in HeLa (A), Raw264.7 UV-treated (B) whole cell lysates.

IHC description: Immunohistochemical analysis of PKC alpha (pT638) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 5578

Gene ID (Mouse): 18750

Gene ID(Rat):

SwissProt (Human): P17252

SwissProt (Mouse): P20444

SwissProt (Rat): P05696

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.