Purified Rabbit anti-PADI4 Antibody

SKU: DL95954A

Size 1: 100 ul

Synonyms : PADI5; PDI5; Protein-arginine deiminase type-4; HL-60 PAD; Peptidylarginine deiminase IV; Protein-arginine deiminase type IV

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to PADI4

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human PADI4

WB description: Western blot analysis of PADI4 expression in BT474 (A), SKOV3 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of PADI4 staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PADI4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 23569

Gene ID (Mouse):

Gene ID(Rat): 29512

SwissProt (Human): Q9UM07

SwissProt (Mouse):

SwissProt (Rat): O88807

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.