Purified Rabbit anti-p38 (pT180) Antibody

SKU: DL92187A

Size 1: 100 ul

Synonyms : CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; SAPK2a

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, D, Z

Applications: WB, IF/IC, IP

Background: Rabbit polyclonal antibody to p38 (pT180)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p38 (pT180). The exact sequence is proprietary.

WB description: Western blot analysis of p38 (pT180) expression in HepG2 PMA-treated (A), NIH3T3 (B), PC12 (C) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of p38 (pT180) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 1432

Gene ID (Mouse): 26416

Gene ID(Rat):

SwissProt (Human): Q16539

SwissProt (Mouse): P47811

SwissProt (Rat): P70618

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.