Purified Rabbit anti-OCT4 Antibody

SKU: DL90915A

Size 1: 100 ul

Synonyms : OCT3; OCT4; OTF3; POU domain, class 5, transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, D, P

Applications: WB, IHC

Background: Rabbit polyclonal antibody to 41916

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 41916. The exact sequence is proprietary.

WB description: Western blot analysis of OCT4 expression in HeLa (A), A549 (B), WI38 (C), NIH3T3 (D), HEK293T (E), mouse brain (F), rat brain (G) whole cell lysates.

IHC description: Immunohistochemical analysis of OCT4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 5460

Gene ID (Mouse): 18999

Gene ID(Rat):

SwissProt (Human): Q01860

SwissProt (Mouse): P20263

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.