Purified Rabbit anti-Nibrin Antibody

SKU: DL94855A

Size 1: 100 ul

Synonyms : NBS; NBS1; P95; Nibrin; Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Nibrin

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Nibrin. The exact sequence is proprietary.

WB description: Western blot analysis of Nibrin expression in HEK293T (A), A549 (B), Jurkat (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Nibrin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 4683

Gene ID (Mouse): 27354

Gene ID(Rat): 85482

SwissProt (Human): O60934

SwissProt (Mouse): Q9R207

SwissProt (Rat): Q9JIL9

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.