Purified Rabbit anti-Nephrin Antibody

SKU: DL94301A

Size 1: 100 ul

Synonyms : NPHN; Nephrin; Renal glomerulus-specific cell adhesion receptor

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Nephrin

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Nephrin. The exact sequence is proprietary.

WB description: Western blot analysis of Nephrin expression in HeLa (A), HEK293T (B), PC12 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Nephrin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)

Gene ID(Human): 4868

Gene ID (Mouse): 54631

Gene ID(Rat): 64563

SwissProt (Human): O60500

SwissProt (Mouse): Q9QZS7

SwissProt (Rat): Q9R044

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.