Purified Rabbit anti-Kir3.1 (pS185) Antibody

SKU: DL93699A

Size 1: 100 ul

Synonyms : GIRK1; G protein-activated inward rectifier potassium channel 1; GIRK-1; Inward rectifier K(+) channel Kir3.1; Potassium channel, inwardly rectifying subfamily J member 3

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, Mk

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Kir3.1 (pS185)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Kir3.1. The exact sequence is proprietary.

WB description: Western blot analysis of Kir3.1 (pS185) expression in A549 (A), Raw264.7 (B), COS7 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Kir3.1 (pS185) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Kir3.1 (pS185) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 3760

Gene ID (Mouse): 16519

Gene ID(Rat): 50599

SwissProt (Human): P48549

SwissProt (Mouse): P63250

SwissProt (Rat): P63251

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.