Purified Rabbit anti-KIF2B Antibody

SKU: DL96257A

Size 1: 100 ul

Synonyms : Kinesin-like protein KIF2B

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to KIF2B

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human KIF2B

WB description: Western blot analysis of KIF2B expression in U937 (A), mouse lung (B), rat lung (C) whole cell lysates.

IHC description: Immunohistochemical analysis of KIF2B staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of KIF2B staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 84643

Gene ID (Mouse): 73470

Gene ID(Rat): 287624

SwissProt (Human): Q8N4N8

SwissProt (Mouse): Q8C0N1

SwissProt (Rat): Q5XI51

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.