Purified Rabbit anti-JAK2 (pY221) Antibody

SKU: DL98621A

Size 1: 100 ul

Synonyms : Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, D, Mk, P

Applications: WB, IH

Background: Rabbit polyclonal antibody to JAK2 (pY221)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human JAK2 (pY221). The exact sequence is proprietary.

WB description: Western blot analysis of JAK2 (pY221) expression in HepG2 (A), LO2 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of JAK2 (pY221) staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)

Gene ID(Human): 3717

Gene ID (Mouse): 16452

Gene ID(Rat): 24514

SwissProt (Human): O60674

SwissProt (Mouse): Q62120

SwissProt (Rat): Q62689

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.