Purified Rabbit anti-IKB beta (pS23) Antibody

SKU: DL90806A

Size 1: 100 ul

Synonyms : IKBB; TRIP9; NF-kappa-B inhibitor beta; NF-kappa-BIB; I-kappa-B-beta; IkB-B; IkB-beta; IkappaBbeta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to IKB beta (pS23)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human IKB beta. The exact sequence is proprietary.

WB description: Western blot analysis of IKB beta (pS23) expression in HEK293T LPS-treated (A), SP2/0 LPS-treated (B), PC12 LPS-treated (C), HT29 (D), NIH3T3 TNFa-treated (E) whole cell lysates.

IHC description: Immunohistochemical analysis of IKB beta (pS23) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of IKB beta (pS23) staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 4793

Gene ID (Mouse): 18036

Gene ID(Rat):

SwissProt (Human): Q15653

SwissProt (Mouse): Q60778

SwissProt (Rat): Q9JIA3

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.