Purified Rabbit anti-Histone H2B (Butyryl-K20) Antibody

SKU: DL99030A

Size 1: 100 ul

Synonyms : HIST1H2BA; TSH2B; Histone H2B type 1-A; Histone H2B testis; Testis-specific histone H2B; HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f; HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IH

Background: Rabbit polyclonal antibody to Histone H2B (Butyryl-K20)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H2B with a site at Butyryl-K20. The exact sequence is proprietary.

WB description: Western blot analysis of Histone H2B (Butyryl-K20) expression in HEK293T (A), U2OS (B), DLD (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Histone H2B (Butyryl-K20) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)

Gene ID(Human): 255626; 3018; 3017; 8339; 8343; 8344; 8346; 8347

Gene ID (Mouse): 319177; 319178; 319179

Gene ID(Rat): 24829

SwissProt (Human): P33778; P62807

SwissProt (Mouse): P70696; Q64475; Q6ZWY9

SwissProt (Rat): Q00729

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.