Purified Rabbit anti-Histone H2A.X (pS139) Antibody

SKU: DL95657A

Size 1: 100 ul

Synonyms : H2AX; Histone H2AX; H2a/x; Histone H2A.X

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Histone H2A.X (pS139)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Histone H2A.X. The exact sequence is proprietary.

WB description: Western blot analysis of Histone H2A.X (pS139) expression in Hela (A) whole cell lysates.

IHC description: Immunohistochemical analysis of Histone H2A.X (pS139) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500)

Gene ID(Human): 3014

Gene ID (Mouse): 15270

Gene ID(Rat):

SwissProt (Human): P16104

SwissProt (Mouse): P27661

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.