Purified Rabbit anti-GABPB1 Antibody

SKU: DL96353A

Size 1: 100 ul

Synonyms : E4TF1B; GABPB; GABPB2; GA-binding protein subunit beta-1; GABP subunit beta-1; GABPB-1; GABP subunit beta-2; GABPB-2; Nuclear respiratory factor 2; Transcription factor E4TF1-47; Transcription factor E4TF1-53

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to GABPB1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human GABPB1

WB description: Western blot analysis of GABPB1 expression in HepG2 (A), HT29 (B), mouse spleen (C), mouse thymus (D) whole cell lysates.

IHC description: Immunohistochemical analysis of GABPB1 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of GABPB1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 2553

Gene ID (Mouse): 14391

Gene ID(Rat):

SwissProt (Human): Q06547

SwissProt (Mouse): Q00420

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.