Purified Rabbit anti-Frataxin Antibody

SKU: DL95935A

Size 1: 100 ul

Synonyms : FRDA; X25; Frataxin, mitochondrial; Friedreich ataxia protein; Fxn

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Frataxin

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Frataxin

WB description: Western blot analysis of Frataxin expression in Jurkat (A), K562 (B), mouse heart (C), mouse liver (D) whole cell lysates.

IHC description: Immunohistochemical analysis of Frataxin staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Frataxin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 2395

Gene ID (Mouse): 14297

Gene ID(Rat):

SwissProt (Human): Q16595

SwissProt (Mouse): O35943

SwissProt (Rat): D3ZYW7

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.