Purified Rabbit anti-FMR1 Antibody

SKU: DL96108A

Size 1: 100 ul

Synonyms : Fragile X mental retardation protein 1; FMRP; Protein FMR-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to FMR1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human FMR1

WB description: Western blot analysis of FMR1 expression in MCF7 (A), Jurkat (B), mouse kidney (C), mouse liver (D) whole cell lysates.

IHC description: Immunohistochemical analysis of FMR1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of FMR1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 2332

Gene ID (Mouse):

Gene ID(Rat): 24948

SwissProt (Human): Q06787

SwissProt (Mouse): P35922

SwissProt (Rat): Q80WE1

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.