Purified Rabbit anti-Estrogen Receptor alpha Antibody

SKU: DL93406A

Size 1: 100 ul

Synonyms : ESR; NR3A1; Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Estrogen Receptor alpha

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Estrogen Receptor alpha. The exact sequence is proprietary.

WB description: Western blot analysis of Estrogen Receptor alpha expression in HepG2 (A), mouse brain (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Estrogen Receptor alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Estrogen Receptor alpha staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 2099

Gene ID (Mouse): 13982

Gene ID(Rat):

SwissProt (Human): P03372

SwissProt (Mouse): P19785

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.