Purified Rabbit anti-EBP1 Antibody

SKU: DL97936A

Size 1: 100 ul

Synonyms : EBP1; Proliferation-associated protein 2G4; Cell cycle protein p38-2G4 homolog; hG4-1; ErbB3-binding protein 1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to EBP1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human EBP1

WB description: Western blot analysis of EBP1 expression in Hela (A), K562 (B), PC12 (C), NIH3T3 (D), mouse liver (E) whole cell lysates.

IHC description: Immunohistochemical analysis of EBP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of EBP1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/50 - 1/100)

Gene ID(Human): 5036

Gene ID (Mouse): 18813

Gene ID(Rat):

SwissProt (Human): Q9UQ80

SwissProt (Mouse): P50580

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.