Purified Rabbit anti-E2F1 Antibody

SKU: DL92905A

Size 1: 100 ul

Synonyms : RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Mk

Applications: WB, IHC

Background: Rabbit polyclonal antibody to E2F1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human E2F1. The exact sequence is proprietary.

WB description: Western blot analysis of E2F1 expression in MCF7 (A), A549 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of E2F1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 1869

Gene ID (Mouse): 13555

Gene ID(Rat):

SwissProt (Human): Q01094

SwissProt (Mouse): Q61501

SwissProt (Rat): O09139

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.