Purified Rabbit anti-E Cadherin Antibody

SKU: DL90198A

Size 1: 100 ul

Synonyms : CDHE; UVO; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to E Cadherin

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human E Cadherin. The exact sequence is proprietary.

WB description: Western blot analysis of E Cadherin expression in HEK293T (A), A431 (B), MCF7 (C), mouse kidney (D), rat kidney (E) whole cell lysates.

IHC description: Immunohistochemical analysis of E Cadherin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 999

Gene ID (Mouse): 12550

Gene ID(Rat): 83502

SwissProt (Human): P12830

SwissProt (Mouse): P09803

SwissProt (Rat): Q9R0T4

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.