Purified Rabbit anti-DNA Ligase 4 Antibody

SKU: DL95005A

Size 1: 100 ul

Synonyms : DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H

Applications: WB, IHC

Background: Rabbit polyclonal antibody to DNA Ligase 4

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DNA Ligase 4. The exact sequence is proprietary.

WB description: Western blot analysis of DNA Ligase 4 expression in KB (A), MCF7 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of DNA Ligase 4 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 3981

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P49917

SwissProt (Mouse):

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.