Purified Rabbit anti-Cyclin E1 Antibody

SKU: DL90171A

Size 1: 100 ul

Synonyms : CCNE; G1/S-specific cyclin-E1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, D, P

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Cyclin E1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin E1. The exact sequence is proprietary.

WB description: Western blot analysis of Cyclin E1 expression in HEK293T (A), mouse brain (B), PC12 (C), SW626 (D), HeLa (E), K562 (F) whole cell lysates.

IHC description: Immunohistochemical analysis of Cyclin E1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Cyclin E1 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 898

Gene ID (Mouse): 12447

Gene ID(Rat):

SwissProt (Human): P24864

SwissProt (Mouse): Q61457

SwissProt (Rat): P39949

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.