Purified Rabbit anti-Cyclin B1 (pS126) Antibody

SKU: DL90169A

Size 1: 100 ul

Synonyms : CCNB; G2/mitotic-specific cyclin-B1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IP

Background: Rabbit polyclonal antibody to Cyclin B1 (pS126)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.

WB description: Western blot analysis of Cyclin B1 (pS126) expression in HT29 nocodazole-treated (A), Jurkat (B), HeLa (C), Raw264.7 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of Cyclin B1 (pS126) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Gene ID(Human): 891

Gene ID (Mouse): 268697

Gene ID(Rat):

SwissProt (Human): P14635

SwissProt (Mouse): P24860

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.