Purified Rabbit anti-Cullin 1 Antibody

SKU: DL91279A

Size 1: 100 ul

Synonyms : Cullin-1; CUL-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, D, P, Rb

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Cullin 1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cullin 1. The exact sequence is proprietary.

WB description: Western blot analysis of Cullin 1 expression in MCF7 (A), HeLa (B), KNRK (C), Jurkat (D), NIH3T3 (E), PC12 (F) whole cell lysates.

IHC description: Immunohistochemical analysis of Cullin 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Cullin 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 8454

Gene ID (Mouse): 26965

Gene ID(Rat):

SwissProt (Human): Q13616

SwissProt (Mouse): Q9WTX6

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.