Purified Rabbit anti-Collagen 17 alpha 1 Antibody

SKU: DL95348A

Size 1: 100 ul

Synonyms : BP180; BPAG2; Collagen alpha-1(XVII) chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Collagen 17 alpha 1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 17 alpha 1. The exact sequence is proprietary.

WB description: Western blot analysis of Collagen 17 alpha 1 expression in HepG2 (A), NIH3T3 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Collagen 17 alpha 1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)

Gene ID(Human): 1308

Gene ID (Mouse): 12821

Gene ID(Rat):

SwissProt (Human): Q9UMD9

SwissProt (Mouse): Q07563

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.