Purified Rabbit anti-CDK1/2/3 (pT14) Antibody

SKU: DL90203A

Size 1: 100 ul

Synonyms : CDK1; CDC2; CDC28A; CDKN1; P34CDC2; Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK2; CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase; CDK3; CDKN3; Cyclin-dependent kinase 3; Cell division protein kinase 3

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, Mk

Applications: WB, IF/IC

Background: Rabbit polyclonal antibody to CDK1/2/3 (pT14)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDK1/2/3. The exact sequence is proprietary.

WB description: Western blot analysis of CDK1/2/3 (pT14) expression in HT29 hydroxyurea-treated (A), HeLa (B), NIH3T3 (C), A431 (D) whole cell lysates.

IHC description:

IF/ICC description: Immunofluorescent analysis of CDK1/2/3 (pT14) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Gene ID(Human): 1017

Gene ID (Mouse): 12534; 12566

Gene ID(Rat): 54237

SwissProt (Human): P06493; P24941; Q00526

SwissProt (Mouse): P11440; P97377; Q80YP0

SwissProt (Rat): P39951; Q63699

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.